A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions

Engineering dual-function single polypeptide catalysts with two abiotic or biotic catalytic entities (or combinations of both) supporting cascade reactions is becoming an important area enzyme engineering and catalysis. Here we present the development a PluriZyme , TR 2 E efficient native transaminase ( k cat : 69.49±1.77 min -1 ) artificial esterase 3908 to 0.41 activities, integrated into scaffold evaluate its utility in reaction. (pH opt 8.0-9.5; T 60-65 °C) efficiently converts methyl 3-oxo-4-(2,4,5-trifluorophenyl)butanoate 3-( R )-amino-4-(2,4,5-trifluorophenyl)butanoic acid, crucial intermediate for synthesis antidiabetic drugs. The reaction proceeds through conversion β-keto ester acid at hydrolytic site subsequently β-amino (e.e. >99%) site. power was proved set esters, demonstrating potential such designs address bioinspired reactions.